Microbiology Unknowns

In Microbiology last semester, I had a difficult time finding photos of the common positive/negative test results. The textbook results are picture perfect, and didn’t help much when I was trying to differentiate between two very similar organisms.

So, in the hopes of helping future Micro students with their unknown identifications I decided I should photograph some of the tests, innoculations, and streak plates that were performed by me- a student. Not a professional microbiologist. In retrospect, I should’ve opened the streak plates to photograph them but live and learn.

Good luck with your unknown IDs. And enjoy the photos!

Novobiocin Susceptibilty Test (top view) On Mueller-Hinton agar.

Novobiocin Susceptibilty Test (bottom view). Lab-provided S.Aureus (top). S.Aureus isolated from nose swab (bottom). On Mueller-Hinton agar.

Sorry. Forgot what this was. Something gram-negative on EMb agar.

S. Aureus (top view). Isolated from nose swab on DNase agar with methyl green

S. Aureus (bottom view). Isolated from nasal swab on DNase agar with methyl green

S.Aureus. Isolated from nasal swab on blood agar.

S. Aureus On Blood Agar

Klebsiella Pneumoniae (top view) On Xylose Lysine Deoxycholate (XLD) Agar

Klebsiella Pneumoniae (bottom view) On XLD Agar

Klebsiella Pneumoniae (top view) On MacConkey Agar

Klebsiella Pneumoniae (bottom view) On MacConkey Agar

Klebsiella Pneumoniae (top view) On Eosin Methylene Blue Agar

Klebsiella Pneumoniae (bottom view) On Eosin Methylene Blue Agar

Klebsiella Pneumoniae (top view) On Hektoen Enteric Agar.

Klebsiella Pneumoniae (bottom view) On Hektoen Enteric Agar

Micrococcus Luteus (left). Serratia Marcescens (middle). Eschericha Coli (right).

S.Aureus (top). S.Epidermis (bottom) on Staphylococcus Medium 110 agar.

S. Aureus (top). S.Epidermis (bottom) on Mannitol Salt Agar.

S. Saphrophyticus on Mannitol Salt Agar.

Swab from nose(top). Swab from fomite/skin (bottom) on Mannitol Salt Agar.

Swab from my Fomite(skin) on left. Swab from my nose on right. Streaked on Staphylococcus Medium 110.

My API 20E results for K. Pneumoniae

My API 20E results for K. Pneumoniae

My API 20E results for K. Pneumoniae

K. Pneumoniae inoculated in Urea slant.

K. Pneumoniae on Nutrient Agar.

K. Pneumoniae on RDS (Russell Double Sugar) slant.

K. Pneumoniae on KIA (Kligler’s Iron Agar) slant.

S. Aureus and Enterobacter aerogenes on Spirit Blue Agar

Bacillus subtillus and Enterobacter aerogenes on Starch agar

B. subtilis and Enterobacter aerogenes on Skim milk Agar (top view)

B. subtilis and Enterobacter aerogenes on Skim milk Agar (bottom view)